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. 2005 May 21;11(19):2984-9.
doi: 10.3748/wjg.v11.i19.2984.

Protective effect of fufanghuangqiduogan against acute liver injury in mice

Shuang-Ying Gui  1 , Wei Wei, Hua Wang, Li Wu, Wu-Yi Sun, Cheng-Yi Wu
Affiliations

Protective effect of fufanghuangqiduogan against acute liver injury in mice

Shuang-Ying Gui et al. World J Gastroenterol. .

Abstract

Aim: To study the effects and possible mechanisms of fufanghuangqiduogan (FFHQ) in mice with acute liver injury (ALI).

Methods: ALI was successfully induced by injecting carbon tetrachloride (CCl4) intraperitoneally and by tail vein injection of Bacillus Calmette Guerin (BCG) and lipopolysaccharide (LPS) in mice, respectively. Each of the two model groups was divided into normal group, model group, FFHQ (60, 120 and 240 mg/kg) treatment groups, and bifendate treatment group. At the end of the experiment, levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), content of malondialdehyde (MDA), activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) in liver homogenate were measured by biochemical methods. The activities of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 (IL-1) were determined by radio-immunoassay. Hepatic tissue sections were stained with hematoxylin and eosin and examined under a light microscope.

Results: In the two models of ALI, FFHQ (60, 120, 240 mg/kg) was found to significantly decrease the serum transaminase (ALT, AST) activities. Meanwhile, FFHQ decreased MDA contents and upregulated the lower SOD and GSH-px levels in liver homogenate. Furthermore, in immunologic liver injury model, FFHQ decreased levels of TNF-alpha and IL-1 in serum. Histologic examination showed that FFHQ could attenuate the area and extent of necrosis, reduce the immigration of inflammatory cells.

Conclusion: FFHQ had protective effect on liver injury induced by either CCl4 or BCG+LPS in mice, and its mechanisms were related to free radical scavenging, increasing SOD and GSH-px activities and inhibiting the production of proinflammatory mediators.

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Figures

Figure 1
Figure 1
Histologic results of tissues stained with hematoxylin and eosin under light microscope in immunologic ALI mice. A: Normal control group; B: model group; C: FFHQ-treated group.
Figure 2
Figure 2
Histologic results of tissues stained with hematoxylin and eosin under light microscope in chemical ALI in mice. A: Normal control group; B: model group; C: FFHQ-treated group.
See this image and copyright information in PMC

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