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. 2005 Jun;73(6):3734-9.
doi: 10.1128/IAI.73.6.3734-3739.2005.

Protein microarray for profiling antibody responses to Yersinia pestis live vaccine

Bei Li  1 , Lingxiao Jiang, Qifeng Song, Junxin Yang, Zeliang Chen, Zhaobiao Guo, Dongsheng Zhou, Zongmin Du, Yajun Song, Jin Wang, Hongxia Wang, Shouyi Yu, Jian Wang, Ruifu Yang
Affiliations

Protein microarray for profiling antibody responses to Yersinia pestis live vaccine

Bei Li et al. Infect Immun. 2005 Jun.

Abstract

A protein microarray representing 149 Yersinia pestis proteins was developed to profile antibody responses in EV76-immunized rabbits. Antibodies to 50 proteins were detected. There are 11 proteins besides F1 and V antigens to which the predominant antibody response occurred, and these proteins show promise for further evaluation as candidates for subunit vaccines and/or diagnostic antigens.

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Figures

FIG. 1.
FIG. 1.
Profiles of serum antibodies against different proteins. The fluorescence values were normalized, logarithm transformed (base 2), and viewed with TreeView. Genes are listed at right, while the time points of immunization and identification numbers of rabbits tested are listed at the top. The color key shows the normalized absolute fluorescence values. The genes were designated with the CO92 gene definition (e.g., YPO0302).
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