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. 2005 Jun;6(6):496-501.
doi: 10.1631/jzus.2005.B0496.

Suppression of Meloidogyne javanica by antagonistic and plant growth-promoting rhizobacteria

Bin Li  1 , Guan-lin Xie, A Soad, J Coosemans
Affiliations

Suppression of Meloidogyne javanica by antagonistic and plant growth-promoting rhizobacteria

Bin Li et al. J Zhejiang Univ Sci B. 2005 Jun.

Abstract

Four rhizobacteria selected out of over 500 isolates from rhizosphere of the vegetables in China were further studied for suppression of the root-knot nematode and soil-borne fungal pathogens in laboratory and greenhouse in Belgium. They were identified as Brevibacillus brevis or Bacillus subtilis by Biolog test and partial 16s rDNA sequence comparison. They not only inhibited the radial growth of the root-infecting fungi Rhizoctonia solani SX-6, Pythium aphanidermatum ZJP-1 and Fusarium oxysporum f.sp. cucumerinum ZJF-2 in vitro, but also exhibited strong nematicidal activity by killing the second stage larvae of Meloidogyne javanica to varying degrees in the greenhouse. The toxic principles of bacterium B7 that showed the highest juvenile mortality were partially characterized. The active factors were heat stability and resistance to extreme pH values. B7 used either as seed dressing or soil drench significantly reduced the nematode populations in the rhizosphere and enhanced the growth of mungbean plants over the controls in the presence or absence of R. solani.

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Figures

Fig. 1
Fig. 1
Effects of the cell-free culture filtrate of rhizobacterium B7 with different treatments on mortality of M. javanica in vitro 1: King B broth; 2: Boiling at 100 °C for 5 min; 3: Refrigeration at −70 °C for 2 h; 4: NaOH treatment at pH 12.0 for 30 min; 5: HCl treatment at pH 2.0 for 30 min; 6: Ethyl acetate extraction; 7: Hexane extraction; 8: Chloroform extraction; 9: Bacterial filtrate. Means on the bars followed by the same letter are not significantly different at the 0.05 levels by Duncan’s multiple-range test
Fig. 2
Fig. 2
Bacterial colonization in the rhizosphere eight weeks after inoculation with nematode alone or nematode and R. solani under greenhouse conditions Means on the bars followed by the same letter are not significantly different at the 0.05 levels by Duncan’s multiple-range test. N: Inoculated with nematode alone; N+R: Inoculated with root knot nematode and R. solani; B2–8: Rhizobacterial isolates. Populations were counted by serial dilution plating
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