Influence of magnesium on the in vitro synthesis of 24,25-dihydroxyvitamin D3 and 1 alpha, 25-dihydroxyvitamin D3
- PMID: 1591144
Influence of magnesium on the in vitro synthesis of 24,25-dihydroxyvitamin D3 and 1 alpha, 25-dihydroxyvitamin D3
Abstract
The role of magnesium in the regulation of 25(OH)D3-24R-hydroxylase and 25(OH)D3-1 alpha-hydroxylase in vitro remains to be clarified. Using a radiochemical assay, the effect of substrate concentration on both enzymes was measured in rat kidney homogenates as well as the alterations in their kinetic behaviour resulting from changes in the magnesium concentration in the incubation medium. The results showed that magnesium could be a modulator of 24- and 1 alpha-hydroxylases. Variations in magnesium caused alterations in the kinetic behaviour of both enzymes, which altered the synthesis rates of 24,25(OH)2D3 and 1,25(OH)2D3. Moreover, the substrate concentration was clearly involved in the response of both enzymes to the modulation by magnesium.
Similar articles
-
Possible involvement of a magnesium dependent mitochondrial alkaline phosphatase in the regulation of the 25-hydroxyvitamin D3-1 alpha-and 25-hydroxyvitamin D3-24R-hydroxylases in LLC-PK1 cells.Magnes Res. 1994 Dec;7(3-4):169-78. Magnes Res. 1994. PMID: 7540407
-
Synthesis of 25-hydroxyvitamin D3-26,23-lactone but not 24,25-dihydroxyvitamin D3 from 25-hydroxyvitamin D3 in opossum kidney cells treated with 1 alpha, 25-dihydroxyvitamin D3.Horm Metab Res. 1995 Feb;27(2):83-9. doi: 10.1055/s-2007-979914. Horm Metab Res. 1995. PMID: 7759063
-
1,25-Dihydroxyvitamin D3-inducible catabolism of vitamin D metabolites in mouse intestine.Am J Physiol. 1990 Apr;258(4 Pt 1):G557-63. doi: 10.1152/ajpgi.1990.258.4.G557. Am J Physiol. 1990. PMID: 2159220
-
Vitamin D hydroxylases.J Cell Biochem. 1992 May;49(1):4-9. doi: 10.1002/jcb.240490103. J Cell Biochem. 1992. PMID: 1644853 Review.
-
Regulation of vitamin D metabolism.Best Pract Res Clin Endocrinol Metab. 2011 Aug;25(4):531-41. doi: 10.1016/j.beem.2011.05.003. Best Pract Res Clin Endocrinol Metab. 2011. PMID: 21872796 Review.
Cited by
-
Macromineral requirements for maintenance and growth in male and female hair sheep.Front Vet Sci. 2023 Mar 2;10:1032429. doi: 10.3389/fvets.2023.1032429. eCollection 2023. Front Vet Sci. 2023. PMID: 36937008 Free PMC article.
-
Conservation Agriculture Affects Grain and Nutrient Yields of Maize (Zea Mays L.) and Can Impact Food and Nutrition Security in Sub-Saharan Africa.Front Nutr. 2022 Jan 26;8:804663. doi: 10.3389/fnut.2021.804663. eCollection 2021. Front Nutr. 2022. PMID: 35155522 Free PMC article.
-
Bone specific binding sites for 1,25(OH)2D3 in magnesium deficiency.J Physiol Biochem. 2004 Sep;60(3):199-203. doi: 10.1007/BF03167029. J Physiol Biochem. 2004. PMID: 15700766
-
Essential Nutrient Interactions: Does Low or Suboptimal Magnesium Status Interact with Vitamin D and/or Calcium Status?Adv Nutr. 2016 Jan 15;7(1):25-43. doi: 10.3945/an.115.008631. Print 2016 Jan. Adv Nutr. 2016. PMID: 26773013 Free PMC article. Review.
-
An Overview of Different Vitamin D Compounds in the Setting of Adiposity.Nutrients. 2024 Jan 11;16(2):231. doi: 10.3390/nu16020231. Nutrients. 2024. PMID: 38257127 Free PMC article. Review.