Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2004;1(3):137-145.
doi: 10.7150/ijms.1.137. Epub 2004 Aug 5.

Anti-tumorigenic and Pro-apoptotic effects of CKBM on gastric cancer growth in nude mice

Vivian Yvonne Shin  1 , Wallace Hau-Leung So, Edgar Shiu-Lam Liu, Ying-Jye Wu, Shiu-Fun Pang, Chi-Hin Cho
Affiliations

Anti-tumorigenic and Pro-apoptotic effects of CKBM on gastric cancer growth in nude mice

Vivian Yvonne Shin et al. Int J Med Sci. 2004.

Abstract

Natural botanical products can be integrated with western medicine to optimize the treatment outcome, increase immune function and minimize the side effects from western drug treatment. CKBM is a combination of herbs and yeasts formulated based on traditional Chinese medicinal principles. Previous study has demonstrated that CKBM is capable of improving immune responsiveness through the induction of cytokine mediators, such as TNF-alpha and IL-6. In this study, we aimed to investigate the effect of this immunomodulatory drug on gastric cancer growth using a human xenograft model. Gastric cancer tissues were implanted subcutaneously into athymic nude mice followed by a 14-day or 28-day of CKBM treatment. Results showed that higher doses of CKBM (0.4 or 0.8 ml/mouse/day) produced a dose-dependent inhibitory effect on gastric tumor growth after 28-day drug treatment. This was associated with a decrease of cellular proliferation by 30% with concomitant increase in apoptosis by 97% in gastric tumor cells when compared with the control group. In contrast, CKBM showed no effect on angiogenesis in gastric tumors. This study demonstrates the anti-tumorigenic action of CKBM on gastric cancer probably via inhibition of cell proliferation and induction of apoptosis, and provides future potential targets of this drug candidate on cancer therapy.

PubMed Disclaimer

Conflict of interest statement

Conflict of interest: Edgar Shiu-Lam Liu, Ying-Jye Wu, and Shiu-Fun Pang work for CK Life Sciences Limited. Others: none declared.

Figures

Figure 1
Figure 1
Effect of CKBM treatment (0.2, 0.4 or 0.8 ml/mouse, given i.g. once daily) for (A) 14 days; (B) 28 days on gastric tumor growth in Balb/c nude mice (*P<0.05 when compared with the corresponding control group). Values are means ± SEM of 10-14 mice per group.
Figure 2
Figure 2
Effect of CKBM treatment (0.2, 0.4 or 0.8 ml/mouse, given i.g. once daily) for (A) 14 days; (B) 28 days on number of blood vessels in gastric tumors (*P<0.05, **P<0.01 when compared with the corresponding control group). Values are means ± SEM of 4-14 mice per group.
Figure 3
Figure 3
Effect of CKBM treatment (0.2, 0.4 or 0.8 ml/mouse, given i.g. once daily) for (A) 14 days; (B) 28 days on number of apoptotic cells in gastric tumors (*P<0.01 when compared with the control group).. Values are means ± SEM of 4-13 mice per group.
Figure 4
Figure 4
Effect of CKBM treatment (0.2, 0.4 or 0.8 ml/mouse, given i.g. once daily) for (A) 14 days; (B) 28 days on number of proliferative cells in gastric tumors (*P<0.05, **P<0.01 when compared with the corresponding control group). Values are means ± SEM of 4-13 mice per group.
See this image and copyright information in PMC

References

    1. Hsieh TC, Lu X, Guo J, Xiong W, Kunicki J, Darzynkiewicz Z, Wu JM. Effects of herbal preparation Equiguard on hormone-responsive and hormone-refractory prostate carcinoma cells: mechanistic studies. Int J Oncol. 2002;20:681–9. - PubMed
    1. Yasukawa K, Ikeya Y, Mitsuhashi H, Iwasaki M, Aburada M, Nakagawa S, Takeuchi M, Takido M. Gomisin A inhibits tumor promotion by 12-O-tetradecanoylphorbol-13acetate in two-stage carcinogenesis in mouse skin. Oncology. 1992;49:68–71. - PubMed
    1. Chang YS, Seo EK, Gyllenhaal C, Block KI. Panax ginseng: a role in cancer therapy? Integr Cancer Ther. 2003;2:13–33. - PubMed
    1. Shin JY, Song JY, Yun YS, Yang HO, Rhee DK, Pyo S. Immunostimulating effects of acidic polysaccharides extract of Panax ginseng on macrophage function. Immunopharmacol Immunotoxicol. 2002;24:469–82. - PubMed
    1. Zhou JR, Yu L, Mai Z, Blackburn GL. Combined inhibition of estrogen-dependent human breast carcinoma by soy and tea bioactive components in mice. Int J Cancer. 2004;108:8–14. - PMC - PubMed