Projections to bimodal sites in the torus semicircularis of the toadfish, Opsanus tau

Abstract

Bimodal cells in the torus semicircularis of the toadfish respond to both directional acoustic stimuli and hydrodynamic stimuli. Our previous physiological work indicated that bimodal cells may be distributed throughout the torus semicircularis. In this study, neurobiotin was used to compare the distribution of auditory-only and bimodal sites and to assess the inputs to those sites. A brief neurobiotin injection with short survival time was used to document the recording location. In other fish, a longer injection and survival time was used at an auditory-only or a bimodal site to fill the axons of the medullary inputs. Auditory-only sites were located in the most dorsal and medial sites in nucleus centralis. Bimodal sites were identified within both nucleus centralis and nucleus ventrolateralis. The greatest number of retrogradely filled cell bodies was found in the descending octaval nucleus following injection at auditory-only recording sites in nucleus centralis. In contrast, retrogradely filled cell bodies were found in both the descending octaval nucleus and the lateral line nucleus medialis following injection at bimodal sites in nucleus centralis or nucleus ventrolateralis. Retrogradely filled cell bodies were located in the dorsal and ventral divisions of the secondary octaval population from injections at either bimodal or auditory-only sites. The secondary octaval population has been implicated in auditory processing based on previous studies of both auditory specialist and auditory generalist fishes; however, this study is the first to reveal the potential role of the secondary octaval population in directional hearing in a fish. Relatively large numbers of retrogradely filled cells around the lateral lemniscus at consistent locations in the medulla indicate that a perilemniscal cell group also might be a component of the directional hearing circuit.