Trace metal concentration, antioxidant enzyme activities and susceptibility to oxidative stress in the tricoptera larvae Hydropsyche exocellata from the Llobregat river basin (NE Spain)

Abstract

Caddisfly larvae of Hydropsyche exocellata were sampled from seven locations receiving increasing levels of urban and industrial waste water discharges along the Llobregat river system (NE Spain) during spring and summer 2003. Locations were selected to include aquatic communities in poor and good ecological state according to measured physicochemical water parameters and the analysis of benthic macroinvertebtrate communities. Whole body residues of selected metals (Fe, Al, Zn, Cu, Co, Ni, Pb, Cd) were determined in conjunction with antioxidant enzyme activities (superoxide dismutase, SOD; catalase, CAT; glutathione peroxidase activity of GST, GSTPX), a phase II enzyme (glutathione-S-transferase, GST) and lipid peroxide levels measured as thiobarbituric reactive species (TBARs) with the aim of investigating whether resident macroinvertebrate benthic species were responsive to changes in water quality. Caddisfly larvae inhabiting those rivers were exposed to increasing levels of metal pollution. Enhanced activities of two (CAT and GST) out of the four tested enzymes, coupled with increased levels of TBARs, indicated increasing levels of stress in the studied species towards downstream reaches or locations near industrial and urban areas. These results indicate that combination of chemical and biochemical responses can be used to assess and diagnose pollution in high stressed river ecosystems.