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. 2005 Jun;49(6):2550-3.
doi: 10.1128/AAC.49.6.2550-2553.2005.

ErmB determinants and Tn916-Like elements in clinical isolates of Clostridium difficile

Patrizia Spigaglia  1 , Valentina Carucci, Fabrizio Barbanti, Paola Mastrantonio
Affiliations

ErmB determinants and Tn916-Like elements in clinical isolates of Clostridium difficile

Patrizia Spigaglia et al. Antimicrob Agents Chemother. 2005 Jun.

Abstract

Erythromycin and tetracycline resistance was analyzed in 37 Clostridium difficile clinical isolates. Strains of different clonal origins showed different erythromycin and tetracycline resistance determinants and different genetic arrangements of the elements. In strains of recent isolation, the presence of Tn916-like elements, never found before in C. difficile clinical isolates, has been demonstrated.

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Figures

FIG. 1.
FIG. 1.
PCR analysis of the genetic organization of the ErmB determinants and the Tn916-like elements. The primers used to analyze the ErmB determinants and the Tn916-like elements were described by Farrow et al. (8) and Wang et al. (25), respectively. The expected amplified fragments are shown as bars in relation to the arrangements of the elements from C. difficile 630 (GenBank accession no. AF109075), C. perfringens CP592 (GenBank accession no. U18931), and E. faecalis DS16 (GenBank accession no. U09422). The length of the fragments are reported in bp, and the primers used for each amplification are indicated below the bars.
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