Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2005 Jun 6;579(14):3115-8.
doi: 10.1016/j.febslet.2005.04.073.

RNA interference induced by siRNAs modified with 4'-thioribonucleosides in cultured mammalian cells

Shuichi Hoshika  1 , Noriaki Minakawa, Hiroyuki Kamiya, Hideyoshi Harashima, Akira Matsuda
Affiliations
Free article

RNA interference induced by siRNAs modified with 4'-thioribonucleosides in cultured mammalian cells

Shuichi Hoshika et al. FEBS Lett. .
Free article

Abstract

Short interfering RNAs (siRNAs) variously modified with 4'-thioribonucleosides against the Photinus luciferase gene were tested for their induction of the RNA interference (RNAi) activity in cultured NIH/3T3 cells. Results indicated that modifications at the sense-strand were well tolerated for RNAi activity except for full modification with 4'-thioribonucleosides. However, the activity of siRNAs modified at the antisense-strand was dependent on the position and the number of modifications with 4'-thioribonucleosides. Since modifications of siRNAs with 4'-thioribonucleosides were well tolerated in RNAi activity compared with that of 2'-O-methyl nucleosides, 4'-thioribonucleosides might be potentially useful in the development of novel and effective chemically modified siRNAs.

PubMed Disclaimer

Publication types