Plasmid pKC7: a vector containing ten restriction endonuclease sites suitable for cloning DNA segments
- PMID: 159209
- DOI: 10.1016/0378-1119(79)90044-1
Plasmid pKC7: a vector containing ten restriction endonuclease sites suitable for cloning DNA segments
Abstract
Plasmid pKC7, a derivative of pBR322, specifies resistance to both ampicillin and kanamycin. The DNA of this small plasmid (5.8 kb) contains unique sites for insertion of DNA cleaved with ten different restriction endonucleases. A detailed restriction endonuclease cleavage map is presented. The utility of this plasmid for cloning is discussed.
Similar articles
-
[The use of bacteriophage lambda fragments in the construction of plasmid vectors for gene cloning. Included: restriction map of lambda DNA (author's transl)].Postepy Biochem. 1979;25(3):351-71. Postepy Biochem. 1979. PMID: 159438 Polish. No abstract available.
-
The plasmid as a tool for plasmid genetics. II. Isolation of point mutations that affect replication of a ColE1-related plasmid.Genet Res. 1982 Dec;40(3):233-47. doi: 10.1017/s0016672300019121. Genet Res. 1982. PMID: 6298062 No abstract available.
-
A pSC101-derived plasmid which shows no sequence homology to other commonly used cloning vectors.Gene. 1984 Nov;31(1-3):165-71. doi: 10.1016/0378-1119(84)90207-5. Gene. 1984. PMID: 6098521
-
Plasmid pGB301, a new multiple resistance streptococcal cloning vehicle and its use in cloning of a gentamicin/kanamycin resistance determinant.Mol Gen Genet. 1981;182(3):414-21. doi: 10.1007/BF00293929. Mol Gen Genet. 1981. PMID: 6272061
-
Cloning and characterization of a plasmid DNA from anacystis nidulans 6301.Gene. 1982 Sep;19(2):221-4. doi: 10.1016/0378-1119(82)90009-9. Gene. 1982. PMID: 6293931
Cited by
-
Characterization and generation of Escherichia coli adenylate cyclase deletion mutants.J Bacteriol. 1991 May;173(10):3238-42. doi: 10.1128/jb.173.10.3238-3242.1991. J Bacteriol. 1991. PMID: 2022622 Free PMC article.
-
Mutational analysis of meiotic and mitotic centromere function in Saccharomyces cerevisiae.Genetics. 1987 Oct;117(2):203-12. doi: 10.1093/genetics/117.2.203. Genetics. 1987. PMID: 3311877 Free PMC article.
-
Role of the Bradyrhizobium japonicum ntrC gene product in differential regulation of the glutamine synthetase II gene (glnII).J Bacteriol. 1988 Dec;170(12):5452-9. doi: 10.1128/jb.170.12.5452-5459.1988. J Bacteriol. 1988. PMID: 2903856 Free PMC article.
-
The sequence of carnation etched ring virus DNA: comparison with cauliflower mosaic virus and retroviruses.EMBO J. 1986 Dec 1;5(12):3083-90. doi: 10.1002/j.1460-2075.1986.tb04614.x. EMBO J. 1986. PMID: 16453731 Free PMC article.
-
Organization and functional analysis of three T-DNAs from the vitopine Ti plasmid pTiS4.Mol Gen Genet. 1992 Nov;235(2-3):292-303. doi: 10.1007/BF00279373. Mol Gen Genet. 1992. PMID: 1465104
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials