Sin, a stage-specific repressor of cellular differentiation
- PMID: 1592811
- PMCID: PMC206042
- DOI: 10.1128/jb.174.11.3561-3569.1992
Sin, a stage-specific repressor of cellular differentiation
Abstract
Sin is a Bacillus subtilis DNA-binding protein which is essential for competence, motility, and autolysin production but also, if expressed on a multicopy plasmid, is inhibitory to sporulation and alkaline protease synthesis. We have now examined the physiological role of Sin in sporulation and found that this protein specifically represses three stage II sporulation genes (spoIIA, spoIIE, and spoIIG) but not the earlier-acting stage 0 sporulation genes. sin loss-of-function mutations cause higher expression of stage II genes and result in a higher frequency of sporulation, in general. Sin binds to the upstream promoter region of spoIIA in vitro and may thus gate entry into sporulation by directly repressing the transcription of stage II genes. In vivo levels of Sin increase rather than decrease at the time of stage II gene induction, suggesting that posttranslational modification may play a role in downregulation of negative Sin function.
Similar articles
-
The role of negative control in sporulation.Res Microbiol. 1991 Sep-Oct;142(7-8):831-9. doi: 10.1016/0923-2508(91)90062-f. Res Microbiol. 1991. PMID: 1664536 Review.
-
Roles of rpoD, spoIIF, spoIIJ, spoIIN, and sin in regulation of Bacillus subtilis stage II sporulation-specific transcription.J Bacteriol. 1992 Jun;174(11):3570-6. doi: 10.1128/jb.174.11.3570-3576.1992. J Bacteriol. 1992. PMID: 1592812 Free PMC article.
-
The Bacillus subtilis SinR protein is a repressor of the key sporulation gene spo0A.J Bacteriol. 1995 Aug;177(16):4619-27. doi: 10.1128/jb.177.16.4619-4627.1995. J Bacteriol. 1995. PMID: 7642487 Free PMC article.
-
Sporulation regulatory protein GerE from Bacillus subtilis binds to and can activate or repress transcription from promoters for mother-cell-specific genes.J Mol Biol. 1992 Aug 20;226(4):1037-50. doi: 10.1016/0022-2836(92)91051-p. J Mol Biol. 1992. PMID: 1518043
-
Control of the cell-specificity of sigma F activity in Bacillus subtilis.Philos Trans R Soc Lond B Biol Sci. 1996 Apr 29;351(1339):537-42. doi: 10.1098/rstb.1996.0052. Philos Trans R Soc Lond B Biol Sci. 1996. PMID: 8735276 Review.
Cited by
-
Postexponential regulation of sin operon expression in Bacillus subtilis.J Bacteriol. 2002 Jan;184(2):564-71. doi: 10.1128/JB.184.2.564-571.2002. J Bacteriol. 2002. PMID: 11751836 Free PMC article.
-
Bacillus subtilis SalA (YbaL) negatively regulates expression of scoC, which encodes the repressor for the alkaline exoprotease gene, aprE.J Bacteriol. 2004 May;186(10):3056-64. doi: 10.1128/JB.186.10.3056-3064.2004. J Bacteriol. 2004. PMID: 15126467 Free PMC article.
-
flaD (sinR) mutations affect SigD-dependent functions at multiple points in Bacillus subtilis.J Bacteriol. 1996 Nov;178(22):6640-3. doi: 10.1128/jb.178.22.6640-6643.1996. J Bacteriol. 1996. PMID: 8932324 Free PMC article.
-
Bacillus subtilis sporulation: regulation of gene expression and control of morphogenesis.Microbiol Rev. 1993 Mar;57(1):1-33. doi: 10.1128/mr.57.1.1-33.1993. Microbiol Rev. 1993. PMID: 8464402 Free PMC article. Review.
-
Analysis of tnrA alleles which result in a glucose-resistant sporulation phenotype in Bacillus subtilis.J Bacteriol. 2000 Sep;182(17):5009-12. doi: 10.1128/JB.182.17.5009-5012.2000. J Bacteriol. 2000. PMID: 10940050 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources