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. 2005 Jun 7;11(21):3217-21.
doi: 10.3748/wjg.v11.i21.3217.

Expression of some tumor associated factors in human carcinogenesis and development of gastric carcinoma

Ming-Dong Zhao  1 , Xue-Mei Hu, Dian-Jing Sun, Qun Zhang, Yu-Hao Zhang, Wei Meng
Affiliations

Expression of some tumor associated factors in human carcinogenesis and development of gastric carcinoma

Ming-Dong Zhao et al. World J Gastroenterol. .

Abstract

Aim: To study the effect of IGF-1/IGF-1R and gastrin/CCK-BR on carcinogenesis and development of human gastric carcinoma and to explore its mechanism and provide a credible theoretical foundation for early diagnosis and molecular therapy of gastric carcinoma.

Methods: mRNA expression levels of IGF-1/IGF-1R and gastrin/CCK-BR were assessed by RT-PCR method in gastric cancer tissues, adjacent mucosa, and tumor-free tissues from 56 patients with gastric carcinoma and normal gastric mucosae from 56 healthy controls. Tissue specimens were obtained by biopsy and confirmed by histological evaluation.

Results: The mRNA levels of IGF-1/IGF-1R were increased in gastric cancer tissues compared with normal tissues from healthy controls and successively increased in tumor-free tissues, adjacent mucosa, and gastric cancer tissues. The mRNA levels of gastrin/CCK-BR were increased in gastric cancer tissues compared with normal tissues from healthy controls. There was a significant difference between gastric cancer tissues and adjacent mucosa and tumor-free tissues, but the mRNA levels of gastrin were not significantly increased in adjacent mucosa and gastric cancer tissues compared with tumor-free tissues. The mRNA levels of CCK-BR were increased in gastric cancer tissues and adjacent mucosa compared with tumor-free tissues, but not significantly increased in adjacent mucosa and gastric cancer tissues compared with gastric cancer tissues.

Conclusion: Overexpression of IGF-1/IGF-1R and gastrin/CCK-BR promotes the disorderly proliferation of gastric mucosa epithelia and it is of great significance in the carcinogenesis and development of gastric carcinoma.

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Figures

Figure 1
Figure 1
mRNA expression of IGF-1/IGF-1R and gastrin/CCK-BR in gastric cancer tissues and normal mucosa from control bP<0.01 vs control.
Figure 2
Figure 2
RT-PCR amplified products of IGF-1/β-actin. A: IGF-1R/β-actin; B: gastrin/β-actin; C: and CCK-BR/β-actin; D: detected by electrophoresis on an 1.8% agarose gel. Lane 1: gastric cancer tissues; lane 2: adjacent mucosa; lane 3: tumor-free tissues; lane 4: healthy controls; M: standard molecular weight of DNA.
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