Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2005 Jun 7;11(21):3222-6.
doi: 10.3748/wjg.v11.i21.3222.

Relationship between matrix metalloproteinase-2 mRNA expression and clinicopathological and urokinase-type plasminogen activator system parameters and prognosis in human gastric cancer

Feng Ji  1 , Yue-Liang Chen, En-Yun Jin, Wei-Lin Wang, Zi-Li Yang, You-Ming Li
Affiliations

Relationship between matrix metalloproteinase-2 mRNA expression and clinicopathological and urokinase-type plasminogen activator system parameters and prognosis in human gastric cancer

Feng Ji et al. World J Gastroenterol. .

Abstract

Aim: To investigate the relationship between matrix metalloproteinase-2 (MMP-2) mRNA expression and clinicopathologic and urokinase-type plasminogen activator (uPA) system parameter and prognosis in human gastric cancer.

Methods: Expression of MMP-2 mRNA, uPA, and uPA-R mRNA in tumor tissues and > or =5 cm adjacent normal tissues from 67 cases of gastric cancer was studied using RT-PCR and Northern blot respectively. Survival analyses were done using the Kaplan-Meier method.

Results: The expression rates of MMP-2 mRNA, uPA and uPA-R mRNA in tumor tissues (31%, 41%, and 51%, respectively) were significantly higher than those in > or =5 cm adjacent tissues (19%, 11%, and 9%; chi(2) = 4.59, 43.58, and 53.24 respectively, P<0.05, 0.0001, and 0.0001, respectively). Expression of MMP-2 mRNA was significantly correlated with lymph node metastasis (metastasis: 61.9%, no metastasis: 39.1%, chi(2) = 7.61, P<0.05), Lauren's classification of diffuse/mixed types: 54.2%, intestinal type: 26.3%, chi(2) = 4.25, P<0.05, expression of uPA and uPA-R mRNA (uPA+: 55.1%, uPA-: 22.2% and uPA-R+: 54.9%, uPA-R-: 18.8%, chi(2) = 5.72 and 6.40 respectively, P<0.05). Kaplan-Meier survival analysis of MMP-2 mRNA expression did not show significant difference in all 67 cases, but revealed an association of the expression of MMP-2 mRNA, uPA, and uPA-R mRNA with worse prognosis (P = 0.0083, 0.0160, and 0.0094, respectively).

Conclusion: MMP-2 may play an important role in the development of invasion and metastasis of gastric cancer.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Expression of MMP-2 mRNA in gastric cancerous tissues and tumor adjacent tissues. Lane M: DNA marker; lanes 1 and 2: gastric cancerous tissues where MMP-2 mRNA is overexpressed; lanes 3 and 4: tumor adjacent tissues; lane 5: normal control.
Figure 2
Figure 2
Expression of uPA and uPA-R mRNA in gastric cancerous tissues and tumor adjacent tissues. Lanes 1 and 4: normal controls; lanes 2 and 5: tumor adjacent tissues; lanes 3 and 6: gastric cancerous tissues where uPA and uPA-R mRNA are overexpressed; lanes 7: empty control.
Figure 3
Figure 3
No significant differences between positive cases and negative cases in Kaplan–Meier survival analysis of MMP-2 mRNA.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Allgayer H, Babic R, Grützner KU, Beyer BC, Tarabichi A, Schildberg FW, Heiss MM. Tumor-associated proteases and inhibitors in gastric cancer: analysis of prognostic impact and individual risk protease patterns. Clin Exp Metastasis. 1998;16:62–73. - PubMed
    1. Yamashita K, Tanaka Y, Mimori K, Inoue H, Mori M. Differential expression of MMP and uPA systems and prognostic relevance of their expression in esophageal squamous cell carcinoma. Int J Cancer. 2004;110:201–207. - PubMed
    1. Schmalfeldt B, Prechtel D, Härting K, Späthe K, Rutke S, Konik E, Fridman R, Berger U, Schmitt M, Kuhn W, et al. Increased expression of matrix metalloproteinases (MMP)-2, MMP-9, and the urokinase-type plasminogen activator is associated with progression from benign to advanced ovarian cancer. Clin Cancer Res. 2001;7:2396–2404. - PubMed
    1. Siddique K, Yanamandra N, Gujrati M, Dinh D, Rao JS, Olivero W. Expression of matrix metalloproteinases, their inhibitors, and urokinase plasminogen activator in human meningiomas. Int J Oncol. 2003;22:289–294. - PubMed
    1. Allgayer H, Babic R, Beyer BC, Grützner KU, Tarabichi A, Schildberg FW, Heiss MM. Prognostic relevance of MMP-2 (72-kD collagenase IV) in gastric cancer. Oncology. 1998;55:152–160. - PubMed

Substances