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. 2005 Jun 7;11(21):3281-4.
doi: 10.3748/wjg.v11.i21.3281.

Tissue distribution and excretion of 125I-lidamycin in mice and rats

You-Ping Liu  1 , Quan-Sheng Li, Yu-Rong Huang, Chang-Xiao Liu
Affiliations

Tissue distribution and excretion of 125I-lidamycin in mice and rats

You-Ping Liu et al. World J Gastroenterol. .

Abstract

Aim: To investigate the tissue distribution, urinary and fecal excretions of (125)I-lidamycin ((125)I-C-1027) in mice and its biliary excretion in rats.

Methods: The total radioactivity assay (RA method) and the radioactivity assay after precipitation with 200 mL/L trichloroacetic acid (TCA-RA method) were used to determine the tissue distribution, and the urinary and fecal excretions of (125)I-C-1027 in mice and its biliary excretion in rats.

Results: Tissue concentrations reached the peak at the fifth minute after administration of (125)I-C-1027 to mice. The highest concentration was in kidney, and the lowest in brain at all test-time points. The organs of the concentrations of (125)I-C-1027 from high to low were kidney, lung, liver, stomach, spleen, uterus, ovary, intestine, muscle, heart, testis, fat, and brain in mice. The accumulative excretion amounts of 0-24 h, and 0-96 h after administration of (125)I-C-1027 were 68.36 and 71.64% in urine, and 2.60 and 3.21% in feces of mice, respectively, and the accumulative excretion amount of 0-24 h was 3.57% in bile in rats.

Conclusion: Our results reflect the characteristics of the tissue distribution, urinary and fecal excretions of (125)I-C-1027 in mice and the biliary excretion of (125)I-C-1027 and its metabolites in rats, and indicate that (125)I-C-1027 and its metabolites are mainly distributed in kidney, and excreted in urine.

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Figures

Figure 1
Figure 1
Tissue distribution of radioactivity of 125I-C-1027 (50 ìg/kg) after i.v. injection to mice (n = 6). A: RA method; and B: TCA-RA method. Values were expressed as mean±SD.
Figure 2
Figure 2
Excretion curves after iv. injection of 125I-C-1027 (50 μg/kg) to mice by RA method (n = 6). A: excretion in urine; and B: excretion in feces. Values were expressed as mean±SD.
Figure 3
Figure 3
Bile excretion curve after i.v. injection of 125I-C-1027 (50 μg/kg) to rats by RA method (n = 5). Values were expressed as mean±SD.
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