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. 2005 Jun;61(Pt 6):664-70.
doi: 10.1107/S0907444905000028. Epub 2005 May 26.

High-throughput crystal-optimization strategies in the South Paris Yeast Structural Genomics Project: one size fits all?

Nicolas Leulliot  1 , Lionel Trésaugues, Michael Bremang, Isabelle Sorel, Nathalie Ulryck, Marc Graille, Ilham Aboulfath, Anne Poupon, Dominique Liger, Sophie Quevillon-Cheruel, Joël Janin, Herman van Tilbeurgh
Affiliations

High-throughput crystal-optimization strategies in the South Paris Yeast Structural Genomics Project: one size fits all?

Nicolas Leulliot et al. Acta Crystallogr D Biol Crystallogr. 2005 Jun.

Abstract

Crystallization has long been regarded as one of the major bottlenecks in high-throughput structural determination by X-ray crystallography. Structural genomics projects have addressed this issue by using robots to set up automated crystal screens using nanodrop technology. This has moved the bottleneck from obtaining the first crystal hit to obtaining diffraction-quality crystals, as crystal optimization is a notoriously slow process that is difficult to automatize. This article describes the high-throughput optimization strategies used in the Yeast Structural Genomics project, with selected successful examples.

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