A comparative autoradiographic study of the density of [3H]SR95531, [3H]MK-801 and [3H]cGMP binding in the locus coeruleus and central pontine grey of spontaneously hypertensive and Wistar-Kyoto rats

Abstract

The Spontaneously Hypertensive rat (SHR) has been previously shown to have a host of neurochemical differences compared with their normotensive counterpart, the Wistar-Kyoto (WKY) rat. Using quantitative receptor autoradiography, the density of GABA(A) and NMDA receptors and [3H]cGMP binding within the locus coeruleus (LC) and central pontine grey (CGPn) were compared in the SHR and WKY rat using the radioligands [3H]SR95531, [3H]MK-801 and [3H]cGMP respectively. It was found that [3H]SR95531 binding was significantly greater in both the LC and CGPn of the SHR compared with the WKY rat (unpaired t test; P < 0.05). Greater binding densities of [3H]MK-801 and [3H]cGMP were also observed in the LC of the SHR compared with the WKY rat; however, no differences in the binding density of these two ligands were observed in the CGPn. It is suggested that these neurochemical differences within the LC of the SHR may relate to phenotypic differences between SHR and WKY rats that have previously been reported.