Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 May 11;20(9):2257-63.
doi: 10.1093/nar/20.9.2257.

Determination of the DNA sequence recognized by the bHLH-zip domain of the N-Myc protein

R Alex  1 , O Sözeri, S Meyer, R Dildrop
Affiliations
Free PMC article

Determination of the DNA sequence recognized by the bHLH-zip domain of the N-Myc protein

R Alex et al. Nucleic Acids Res. .
Free PMC article

Abstract

The DNA-binding domain of the murine N-Myc protein, comprising the basic helix-loop-helix-zipper (bHLH-zip) region was expressed as a fusion protein in E. coli. The affinity purified glutathione-S-transferase-N-Myc fusion protein (GST-N-MYC) was used to select the N-Myc specific DNA-recognition motif from a pool of random-sequence oligonucleotides. After seven rounds of binding-site selection, specifically enriched oligonucleotides were cloned and sequenced. Of 31 individual oligonucleotides whose sequences were determined, 30 contained a common DNA-motif, defining the hexameric consensus sequence CACGTG. We confirm by mutational analysis that binding of the N-Myc derived bHLH-zip domain to this motif is sequence-specific.

PubMed Disclaimer

References

    1. Nucleic Acids Res. 1991 Jan 11;19(1):184 - PubMed
    1. Cell. 1989 Mar 10;56(5):777-83 - PubMed
    1. Science. 1991 Mar 8;251(4998):1211-7 - PubMed
    1. Science. 1990 Nov 23;250(4984):1149-51 - PubMed
    1. Genes Dev. 1990 Sep;4(9):1454-61 - PubMed

Publication types