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Comparative Study
. 2005 Jun;22(6):972-80.
doi: 10.1007/s11095-005-4592-1. Epub 2005 Jun 8.

N4,N9-dioleoyl spermine is a novel nonviral lipopolyamine vector for plasmid DNA formulation

Osama A A Ahmed  1 , Noppadon Adjimatera, Charareh Pourzand, Ian S Blagbrough
Affiliations
Comparative Study

N4,N9-dioleoyl spermine is a novel nonviral lipopolyamine vector for plasmid DNA formulation

Osama A A Ahmed et al. Pharm Res. 2005 Jun.

Abstract

Purpose: To study the effect of synthesized N4,N9-dioleoyl spermine on DNA condensation and then measure its transfection efficiency in cell culture.

Methods: The lipopolyamine was synthesized from the naturally occurring polyamine spermine. The ability of this novel compound to condense DNA was studied using ethidium bromide fluorescence quenching and light scattering assays. Transfection efficiency was studied in primary skin cells (FEK4) and in an immortalized cancer cell line (HtTA), and compared with the commercially available transfection formulations Lipofectin and Lipofectamine.

Results: The synthesized N4,N9-dioleoyl spermine formula is efficient at condensing calf thymus and circular plasmid DNA and effectively transfects both primary skin cells and cancer cell lines at low charge ratios of (+/- ammonium/phosphate) 2.5.

Conclusions: N4,N9-Dioleoyl spermine condenses DNA and achieves high transfection levels in cultured cells.

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References

    1. J Membr Biol. 2003 Jan 15;191(2):99-112 - PubMed
    1. Gene Ther. 2003 Feb;10 (3):261-71 - PubMed
    1. Bioconjug Chem. 2001 Mar-Apr;12 (2):251-7 - PubMed
    1. Biochemistry. 2001 Nov 13;40(45):13644-51 - PubMed
    1. Chem Commun (Camb). 2001 Nov 7;(21):2234-5 - PubMed

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