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. 2005 Jun;43(6):2624-8.
doi: 10.1128/JCM.43.6.2624-2628.2005.

Distribution of nocardia species in clinical samples and their routine rapid identification in the laboratory

Georges Wauters  1 , Véronique Avesani, Jacqueline Charlier, Michèle Janssens, Mario Vaneechoutte, Michel Delmée
Affiliations

Distribution of nocardia species in clinical samples and their routine rapid identification in the laboratory

Georges Wauters et al. J Clin Microbiol. 2005 Jun.

Abstract

Eighty-six Nocardia strains isolated from clinical samples in Belgium were identified by 16S rRNA gene sequencing. Eighty-three (96%) strains belonged to only six Nocardia species: N. farcinica (38 [44%]), N. nova (19 [22%]), N. cyriacigeorgica (13 [15%]), N. brasiliensis (6 [6.9%]), N. abscessus (5 [5.8%]), and N. paucivorans (2 [2.3%]). A gallery of nine conventional and enzymatic tests was developed for the rapid identification of the most common species isolated during this survey. Pyrrolidonyl aminopeptidase, gamma-glutamyl aminopeptidase, alpha-mannosidase, and alpha-glucosidase were found to be highly discriminating and could be used to develop an identification scheme.

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Figures

FIG. 1.
FIG. 1.
Algorithm for the identification of the six most relevant species isolated in this study.
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