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. 2005 Jun;43(6):2994-5.
doi: 10.1128/JCM.43.6.2994-2995.2005.

Frequency of sample submission for optimal utilization of the cell culture cytotoxicity assay for detection of Clostridium difficile toxin

Anita P Borek  1 , Deborah Z Aird, Karen C Carroll
Affiliations

Frequency of sample submission for optimal utilization of the cell culture cytotoxicity assay for detection of Clostridium difficile toxin

Anita P Borek et al. J Clin Microbiol. 2005 Jun.

Abstract

We reviewed the results of repeated sample submissions within a 7-day time frame for Clostridium difficile toxin testing. A total of 2,940 samples were tested during a 3-month period using a cell culture cytotoxicity assay (CCCA). The results from all second samples (n = 1,101) were concordant with the original test result. In only two cases (0.8%; n = 247) was a third sample positive when the first two samples were negative. In this study, submission of multiple samples for CCCA did not increase detection of Clostridium difficile infection.

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