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. 2005 Jun 21;11(23):3601-4.
doi: 10.3748/wjg.v11.i23.3601.

Co-expression of heat shock protein 70 and glucose-regulated protein 94 in human gastric carcinoma cell line BGC-823

Xiao-Ping Wang  1 , Jing Liao, Guo-Zhen Liu, Xing-Cui Wang, Hong-Wei Shang
Affiliations

Co-expression of heat shock protein 70 and glucose-regulated protein 94 in human gastric carcinoma cell line BGC-823

Xiao-Ping Wang et al. World J Gastroenterol. .

Abstract

Aim: To investigate the co-expression and significance of heat shock protein 70 (HSP70) and glucose-regulated protein 94 (grp94) in human gastric carcinoma cell line BGC-823.

Methods: The expression and localization of HSP70 and grp94 in human gastric carcinoma cell line BGC-823 were determined by immunocytochemistry and indirect immunofluorescence cytochemical staining. Flow cytometry was used to analyze the correlation between expression of HSP70, grp94 and cell cycle in BGC-823 cell line.

Results: Gastric cancer cell line BGC-823 expressed high level of HSP70 and grp94. The positive rate of HSP70 and grp94 was 84.9+/-4.94% and 79.6+/-5.16%, respectively. Both of them were stained in cell plasma. There was a significant difference compared with control group (1.9+/-0.94%, P<0.01). During the cell cycle, HSP70 and grp94 were continuously expressed in BGC-823.

Conclusion: HSP70 and grp94 are highly expressed in human gastric carcinoma BGC-823 cells through the whole cell cycle. There is no relationship between expression of HSP70, grp94 and cell cycle.

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Figures

Figure 1
Figure 1
Expressions of HSP70 and grp94 in gastric cancer cell line BGC-823 by immunocytochemistry and immunofluorescence cytochemistry, ×400. A: HSP70 positive expression in cytoplasm; B: grp94 immunostaining in cytoplasm; C: HSP70 immunostaining in cytoplasm; D: grp94 green immunofluorescence in cytoplasm.
Figure 2
Figure 2
Expression of grp94 (A) and HSP70 (B) in cell cycle of gastric cancer cell line BGC-823.
See this image and copyright information in PMC

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