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Review
. 2005 Apr-Jun;9(2):320-30.
doi: 10.1111/j.1582-4934.2005.tb00358.x.

Reprogramming mediated by stem cell fusion

Dominic J Ambrosi  1 , Theodore P Rasmussen
Affiliations
Review

Reprogramming mediated by stem cell fusion

Dominic J Ambrosi et al. J Cell Mol Med. 2005 Apr-Jun.

Abstract

Advances in mammalian cloning prove that somatic nuclei can be reprogrammed to a state of totipotency by transfer into oocytes. An alternative approach to reprogram the somatic genome involves the creation of hybrids between somatic cells and other cells that contain reprogramming activities. Potential fusion partners with reprogramming activities include embryonic stem cells, embryonic germ cells, embryonal carcinoma cells, and even differentiated cells. Recent advances in fusion-mediated reprogramming are discussed from the standpoints of the developmental potency of hybrid cells, genetic and epigenetic correlates of reprogramming, and other aspects involved in the reprogramming process. In addition, the utility of fusion-mediated reprogramming for future cell-based therapies is discussed.

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References

    1. Gurdon JB. Adult frogs derived from the nuclei of single somatic cells. Dev Biol. 1962; 4: 256–73. - PubMed
    1. Wilmut I, Schnieke AE, McWhir J, Kind AJ, Campbell KH. Viable offspring derived from fetal and adult mammalian cells. Nature 1997; 385: 810–3. - PubMed
    1. Eggan K, Akutsu H, Loring J, Jackson‐Grusby L, Klemm M, Rideout WM 3rd, Yanagimachi R, Jaenisch R. Hybrid vigor, fetal overgrowth, and viability of mice derived by nuclear cloning and tetraploid embryo complementation. Proc Natl Acad Sci USA. 2001; 98: 6209–14. - PMC - PubMed
    1. Rideout WM 3rd, Wakayama T, Wutz A, Eggan K, Jackson‐Grusby L, Dausman J, Yanagimachi R, Jaenisch R. Generation of mice from wild‐type and targeted ES cells by nuclear cloning. Nat Genet. 2000; 24: 109–10. - PubMed
    1. Jaenisch R Eggan K, Humpherys D, Rideout W, Hochedlinger K. Nuclear cloning, stem cells, and genomic reprogramming. Cloning Stem Cells 2002; 4: 389–96. - PubMed