In vivo neurochemical monitoring and the study of behaviour

Abstract

In vivo neurochemical monitoring techniques measure changes in the extracellular compartment of selected brain regions. These changes reflect the release of chemical messengers and intermediates of brain energy metabolism resulting from the activity of neuronal assemblies. The two principal techniques used in neurochemical monitoring are microdialysis and voltammetry. The presence of glutamate in the extracellular compartment and its pharmacological characteristics suggest that it is released from astrocytes and acts as neuromodulator rather than a neurotransmitter. The changes in extracellular noradrenaline and dopamine reflect their role in the control of behaviour. Changes in glucose and oxygen, the latter a measure of local cerebral blood flow, reflect synaptic processing in the underlying neuronal networks rather than a measure of efferent output from the brain region. In vivo neurochemical monitoring provides information about the intermediate processing that intervenes between the application of the stimulus and the resulting behaviour but does not reflect the final efferent output that leads to behaviour.