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. 1992 Apr;373(4):201-4.
doi: 10.1515/bchm3.1992.373.1.201.

Construction of a plasmid containing the complete coding region of human elongation factor 2

J Hanes  1 , J Freudenstein, G Rapp, K H Scheit
Affiliations

Construction of a plasmid containing the complete coding region of human elongation factor 2

J Hanes et al. Biol Chem Hoppe Seyler. 1992 Apr.

Abstract

A plasmid pUChEF-2 containing the coding sequence as well as the complete 3'-untranslated region (3'UTR) of human EF-2 mRNA was constructed. The plasmid construct was assembled from a cDNA insert of pHGR81 (Rapp et al., (1988) Biol. Chem. Hoppe-Seyler 369, 247-250) comprising the C-terminal portion of the coding region and the 3'UTR, as well as a polymer chain reaction PCR fragment (Rapp et al., (1989) Biol. Chem. Hoppe-Seyler 370, 1071-1075) covering the missing part of the coding region from the amino-terminus.

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