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Comparative Study
. 2005 Oct 3;139(2):357-60.
doi: 10.1016/j.molbrainres.2005.05.009.

RNAi-mediated silencing of leptin gene expression increases cell death in C6 glioblastoma cells

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Comparative Study

RNAi-mediated silencing of leptin gene expression increases cell death in C6 glioblastoma cells

Russell Brown et al. Brain Res Mol Brain Res. .

Abstract

We previously demonstrated that the brain, pituitary, and C6 glioblastoma cells express leptin. To determine the physiological role of brain-derived leptin, we sought to selectively silence its expression using RNA interference (RNAi) in vitro. One of four potential targets, siRNA L7, reduced leptin mRNA by 50% (P < 0.05) and protein by 55% (P < 0.0001) in C6 cells. RNAi also induced a twofold increase in cell death as seen by ethidium homodimer-1 (P < 0.015) and TUNEL (P < 0.005) staining. These data suggest that endogenous leptin may be a critical factor promoting cell survival in the brain.

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