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. 2005 Oct;222(2):301-6.
doi: 10.1007/s00425-005-1524-6. Epub 2005 Jun 21.

DNA methylation increases throughout Arabidopsis development

L Ruiz-García  1 , M T Cervera, J M Martínez-Zapater
Affiliations

DNA methylation increases throughout Arabidopsis development

L Ruiz-García et al. Planta. 2005 Oct.

Abstract

We used amplified fragment length polymorphisms (AFLP) to analyze the stability of DNA methylation throughout Arabidopsis development. AFLP can detect genome-wide changes in cytosine methylation produced by DNA demethylation agents, such as 5-azacytidine, or specific mutations at the DDM1 locus. In both cases, cytosine demethylation is associated with a general increase in the presence of amplified fragments. Using this approach, we followed DNA methylation at methylation sensitive restriction sites throughout Arabidopsis development. The results show a progressive DNA methylation trend from cotyledons to vegetative organs to reproductive organs.

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