Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2005 Jul;73(7):4441-4.
doi: 10.1128/IAI.73.7.4441-4444.2005.

The order of prime-boost vaccination of neonatal calves with Mycobacterium bovis BCG and a DNA vaccine encoding mycobacterial proteins Hsp65, Hsp70, and Apa is not critical for enhancing protection against bovine tuberculosis

Margot A Skinner  1 , D Neil Wedlock, Geoffrey W de Lisle, Michèle M Cooke, Ricardo E Tascon, Jose C Ferraz, Douglas B Lowrie, H Martin Vordermeier, R Glyn Hewinson, Bryce M Buddle
Affiliations

The order of prime-boost vaccination of neonatal calves with Mycobacterium bovis BCG and a DNA vaccine encoding mycobacterial proteins Hsp65, Hsp70, and Apa is not critical for enhancing protection against bovine tuberculosis

Margot A Skinner et al. Infect Immun. 2005 Jul.

Abstract

Priming neonatal calves at birth with a Mycobacterium bovis bacillus Calmette-Guérin (BCG) vaccine and boosting with a DNA vaccine consisting of plasmids encoding mycobacterial antigens Hsp65, Hsp70, and Apa or the reverse prime-boost sequence induced similar levels of protection against experimental challenge with Mycobacterium bovis. When M. bovis was isolated from a thoracic lymph node following challenge, the two groups of calves given the prime-boost regimen had significantly lower numbers of M. bovis isolates than those vaccinated with BCG alone. These observations suggest that the exact sequence of administration of a prime-boost vaccination regimen in a neonatal animal model is not critical to the development of immunity.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
IFN-γ (A) and IL-2 (B) released from bovine PPD-stimulated whole blood cultures from animals vaccinated with BCG at 24 to 48 h of age and DNA vaccine at 6 and 9 weeks of age (▴), BCG at 24 to 48 h of age (•), and DNA vaccine at 24 to 48 h of age and at 3 weeks of age and BCG at 6 weeks of age (▪) and from animals that were not vaccinated (○). IFN-γ levels are presented as mean concentrations (ng/ml) and IL-2 levels as mean stimulation indices (SI). The error bars represent standard errors of the means. Calves were challenged with M. bovis at 17 to 18 weeks of age.
See this image and copyright information in PMC

References

    1. Buddle, B. M., G. W. de Lisle, A. Pfeffer, and F. E. Aldwell. 1995. Immunological responses and protection against Mycobacterium bovis in calves vaccinated with a low dose of BCG. Vaccine 13:1123-1130. - PubMed
    1. Buddle, B. M., J. M. Pollock, M. A. Skinner, and D. N. Wedlock. 2003. Development of vaccines to control bovine tuberculosis in cattle and relationship to vaccine development for other intracellular pathogens. Int. J. Parasitol. 33:555-566. - PubMed
    1. Buddle, B. M., D. N. Wedlock, N. A. Parlane, L. A. L. Corner, G. W. de Lisle, and M. A. Skinner. 2003. Revaccination of neonatal calves with Mycobacterium bovis BCG reduced the level of protection against tuberculosis induced by a single vaccination. Infect. Immun. 71:6411-6419. - PMC - PubMed
    1. Colditz, G. A., C. S. Berkey, F. Mosteller, T. F. Brewer, M. E. Wilson, E. Burdick, and H. V. Fineberg. 1995. The efficacy of bacillus Calmette-Guérin vaccination of newborns and infants in the prevention of tuberculosis: meta-analyses of published literature. Pediatrics 96:29-35. - PubMed
    1. Feng, C. G., U. Palendira, C. Demangel, J. M. Spratt, A. S. Malin, and W. J. Britton. 2001. Priming with DNA immunization augments protective efficacy of Mycobacterium bovis bacille Calmette-Guérin against tuberculosis. Infect. Immun. 69:4174-4176. - PMC - PubMed

Publication types