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. 2005 Jul;6(7):650-5.
doi: 10.1631/jzus.2005.B0650.

Influence of CO2 pneumoperitoneum on intracellular pH and signal transduction in cancer cells

Li-Ping Cao  1 , Guo-Ping Ding, Ri-Sheng Que, Shu Zheng
Affiliations

Influence of CO2 pneumoperitoneum on intracellular pH and signal transduction in cancer cells

Li-Ping Cao et al. J Zhejiang Univ Sci B. 2005 Jul.

Abstract

Object: The authors studied the influence of CO(2) pneumoperitoneum on intracellular pH and signal transduction arising from cancer cell multiplication in laparoscopic tumor operation.

Method: They set up a simulation of pneumoperitoneum under different CO(2) pressure, and then measured the variation of intracellular pH (pHi) at different time and the activity of protein kinase C (PKC) and protein phosphatase 2a (PP2a) at the end of the pneumoperitoneum. After 1 week, the concentration of cancer cells in the culture medium was calculated.

Result: When the pressure of CO(2) pneumoperitoneum was 0, 10, 20, 30 mmHg respectively, the average pHi was 7.273, 7.075, 6.783, 6.693 at the end of the pneumoperitoneum; PKC activity was 159.4, 168.5, 178.0, 181.6 nmol/(g.min) and PP2a was 4158.3, 4066.9, 3984.0, 3878.5 nmol/(g.min) respectively. After 1 week, the cancer cells concentration was 2.15 x 10(5), 2.03 x 10(5), 2.20 x 10(5), 2.18 x 10(5) L(-1).

Conclusion: CO(2) pneumoperitoneum could promote acidosis in cancer cells, inducing the activation of protein kinase C and deactivation of protein phosphatase 2a, but it could not accelerate the mitosis rate of the cancer cells.

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Figures

Fig. 1
Fig. 1
pHi of cancer cells at different pressure and time
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