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. 2005 Jul;6(7):668-72.
doi: 10.1631/jzus.2005.B0668.

Extracellular HIV Tat and Tat cysteine rich peptide increase CCR5 expression in monocytes

Lin Zheng  1 , Yi-da Yang, Guo-cai Lu, Maria S Salvato
Affiliations

Extracellular HIV Tat and Tat cysteine rich peptide increase CCR5 expression in monocytes

Lin Zheng et al. J Zhejiang Univ Sci B. 2005 Jul.

Abstract

In our previous work we reported that HIV Tat and 6 cysteine rich peptides of Tat induce tumor necrosis factor-related apoptosis-induced ligand (TRAIL) in human monocytes (Yang et al., 2003). Here our results showed that HIV Tat and Tat cysteine rich peptide increase CCR5 expression in human monocytes, and this activity is inhibited by rabbit anti-Tat. Boiled Tat does not increase CCR5 expression in monocytes. These results provide insight into a new mechanism by which HIV Tat plays a key role in the pathogenesis of HIV-1 infection.

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Figures

Fig. 1
Fig. 1
HIV Tat increase CCR5 expression in monocytes. One million monocytes were cultured with 100 nmol/L HIV Tat, at day 0 (a), 1 (b), 3 (c), 5 (d), collected cells and detected CCR5 expression in monocytes using Flow Cytometry analysis. Results shown are representative of three independent experiments. Dotted lines represent unstimulated control cells
Fig. 2
Fig. 2
HIV Tat increases CCR5 expression in monocytes in dose dependent manner. One million monocytes were cultured with [10 nmol/L (b), 100 nmol/L (c), 1000 nmol/L (d)] or without Tat (a) for 5 d, collected cells and detected CCR5 using Flow Cytometry. Results shown are representative of three independent experiments. Dotted lines represent unstimulated control cells
Fig. 3
Fig. 3
HIV Tat specifically increase CCR5 expression in monocytes. HIV Tat alone (a), HIV Tat+rabbit anti-Tat (b), boiled Tat (c) co-cultured with monocytes for 5 d, CCR5 expression was analyzed by Flow Cytometry. Results shown are representative of three independent experiments. Dotted lines represent unstimulated control cells
Fig. 4
Fig. 4
HIV Tat peptide 8 increase CCR5 expression in monocytes. Monocytes were cultured with Tat peptide 1 (a), 8 (b), 12 (c), 13 (d), 19 (e), 25 (f), 31 (g) as indicated in methods and materials, for 5 d and CCR5 expression was analyzed by Flow Cytometry. Results shown are representative of three independent experiment. Dotted lines represent unstimulated control cells
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