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. 2005 Jul 4;579(17):3731-6.
doi: 10.1016/j.febslet.2005.05.069.

Direct interaction between metastasis-associated protein 1 and endophilin 3

Yuko Aramaki  1 , Katsuhiro Ogawa, Yasushi Toh, Takahiro Ito, Nobuyoshi Akimitsu, Hiroshi Hamamoto, Kazuhisa Sekimizu, Kimihiko Matsusue, Akira Kono, Haruo Iguchi, Soichi Takiguchi
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Free article

Direct interaction between metastasis-associated protein 1 and endophilin 3

Yuko Aramaki et al. FEBS Lett. .
Free article

Abstract

The yeast two-hybrid system was used to search for partners of mouse metastasis-associated protein 1 (Mta1). Screening of a cDNA library prepared from mouse embryo yielded positive clones coding for endophilin 3. The site of interaction was suggested to be the SH-3-binding domain of Mta1 and SH-3 domain of endophilin 3. This interaction was confirmed by GST pull-down assay in vitro and immunoprecipitation in vivo. The Mta1 and endophilin 3 transcripts were highly expressed in testis and brain. But, Mta1 localized mainly in nucleus and to a lesser extent in cytoplasm while endophilin 3 localized mainly in cytoplasm. If Mta1 functions in cytoplasm, it might be involved in the regulation of endocytosis mediated by endophilin 3.

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