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. 2005;4(2):8.
doi: 10.1186/jbiol27. Epub 2005 Jun 27.

Movement in ribosome translocation

Christopher S Fraser  1 , John W B Hershey
Affiliations

Movement in ribosome translocation

Christopher S Fraser et al. J Biol. 2005.

Abstract

Translocation of peptidyl-tRNA and mRNA within the ribosome during protein synthesis is promoted by the elongation factor EF-G and by the hydrolysis of GTP. A new study reports that EF-G binds to ribosomes as an EF-G.GDP complex and that GTP is exchanged for GDP on the ribosome. Together with cryo-electron microscopy, this unexpected finding helps clarify the role of GTP in translocation.

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Figures

Figure 1
Figure 1
The pathway of translocation. The tRNAs are shown as colored bars, some with attached amino acids depicted as circles colored to correspond to their tRNAs. The reactions in the pathway are described in the text; the mRNA and bound EF-G are not shown.
See this image and copyright information in PMC

References

    1. Valle M, Zavialov A, Li W, Stagg SM, Sengupta J, Nielsen RC, Nissen P, Harvey SC, Ehrenberg M, Frank J. Incorporation of aminoacyl-tRNA into the ribosome as seen by cryo-electron microscopy. Nat Struct Biol. 2003;10:899–906. doi: 10.1038/nsb1003. - DOI - PubMed
    1. Zavialov A, Buckingham RH, Ehrenberg M. A post-termination ribosomal complex is the guanine nucleotide exchange factor for peptide release factor RF3. Cell. 2001;107:115–124. doi: 10.1016/S0092-8674(01)00508-6. - DOI - PubMed
    1. Zavialov AV, Hauryliuk VV, Ehrenberg M. Guanine-nucleotide exchange on ribosome-bound elongation factor G initiates the translocation of tRNAs. J Biol. 2005;4:9. - PMC - PubMed
    1. Stark H, Rodnina MV, Wieden HJ, van Heel M, Wintermeyer W. Large-scale movement of elongation factor G and extensive conformational change of the ribosome during translocation. Cell. 2000;100:301–309. doi: 10.1016/S0092-8674(00)80666-2. - DOI - PubMed
    1. Agrawal RK, Linde J, Sengupta J, Nierhaus KH, Frank J. Localization of L11 protein on the ribosome and elucidation of its involvement in EF-G-dependent translocation. J Mol Biol. 2001;311:777–787. doi: 10.1006/jmbi.2001.4907. - DOI - PubMed

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