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Clinical Trial
. 2005 Sep;97(2):136-40.
doi: 10.1007/s00436-005-1406-7. Epub 2005 Jun 29.

In vivo and in vitro analysis of chloroquine resistance in Plasmodium falciparum isolates from Senegal

Ousmane Sarr  1 , Alissa Myrick, Johanna Daily, Bernard M Diop, Therese Dieng, Omar Ndir, Pape Salif Sow, Souleymane Mboup, Dyann F Wirth
Affiliations
Clinical Trial

In vivo and in vitro analysis of chloroquine resistance in Plasmodium falciparum isolates from Senegal

Ousmane Sarr et al. Parasitol Res. 2005 Sep.

Abstract

To determine the predictive value of chloroquine (CQ) resistance markers in Senegal, Plasmodium falciparum DNA polymorphisms in pfmdr1and pfcrt were examined in relation to clinical outcome. Despite CQ treatment, 17% of patients had parasitemia after 28 days. Examination of molecular markers of CQ resistance revealed that 64% of all isolates had the T76 resistant allele at the pfcrt locus, while 30% carried the Y86 resistant allele at the pfmdr1 locus. The pfcrt T76 allele was present not only in all in vivo resistant isolates, 89% of in vitro resistant isolates, but also in 35% of in vitro sensitive isolates. The pfmdr1 N86Y polymorphism did not correlate with in vitro or in vivo CQ resistance. Our data suggest that the pfcrt T76 allele alone is required but not a sufficient predictor for in vivo CQ resistance.

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