Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Apr;58(4):1402-7.
doi: 10.1128/aem.58.4.1402-1407.1992.

Cloning, expression, and nucleotide sequence of a lipase gene from Pseudomonas fluorescens B52

Y Tan  1 , K J Miller
Affiliations

Cloning, expression, and nucleotide sequence of a lipase gene from Pseudomonas fluorescens B52

Y Tan et al. Appl Environ Microbiol. 1992 Apr.

Abstract

In this study, we report the cloning and expression of lipase gene from Pseudomonas fluorescens B52, a psychrotrophic spoilage bacterium isolated from refrigerated raw milk. Sequence analysis revealed one major open reading frame of 1,428 nucleotides that was predicted to encode a protein with a molecular weight of 50,241. The predicted enzyme was found to contain an amino acid sequence highly homologous to the putative substrate-binding domain present within all lipases examined to date.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Bacteriol. 1986 May;166(2):385-91 - PubMed
    1. Lipids. 1988 Jul;23(7):701-6 - PubMed
    1. Appl Environ Microbiol. 1987 Jan;53(1):211-3 - PubMed
    1. FEBS Lett. 1988 Dec 19;242(1):36-40 - PubMed
    1. Lipids. 1988 Dec;23(12):1101-6 - PubMed