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. 2005 Jul;43(7):3463-6.
doi: 10.1128/JCM.43.7.3463-3466.2005.

Use of PCR analyses to define the distribution of Ralstonia species recovered from patients with cystic fibrosis

Tom Coenye  1 , Theodore Spilker, Rebecca Reik, Peter Vandamme, John J Lipuma
Affiliations

Use of PCR analyses to define the distribution of Ralstonia species recovered from patients with cystic fibrosis

Tom Coenye et al. J Clin Microbiol. 2005 Jul.

Abstract

Two new PCR assays (for Ralstonia species and Ralstonia respiraculi), together with previously published PCR assays, were used to assess Ralstonia isolates recovered from 111 cystic fibrosis patients. Ralstonia mannitolilytica accounted for 46% of isolates, while R. respiraculi and Ralstonia pickettii accounted for 19% and 18%, respectively. Ralstonia basilensis and Ralstonia metallidurans, species not previously recovered from human samples, were also identified.

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Figures

FIG. 1.
FIG. 1.
PCR analysis of Ralstonia respiraculi, Ralstonia species, and CF-relevant bacteria. (A) PCR using Ralstonia genus-specific primers RalGS-F and RalGS-R. (B) PCR using R. respiraculi-specific primers Rres-F and Rres-R. Lanes M, reference markers; lanes 1, R. mannitolilytica; lanes 2, R. pickettii; lanes 3 to 6, R. respiraculi; lanes 7, R. gilardii; lanes 8, R. insidiosa; lanes 9, R. paucula; lanes 10, R. metallidurans; lanes 11, R. taiwanenesis; lanes 12, R. eutropha; and lanes 13, negative control (water).
See this image and copyright information in PMC

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