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. 2005 Jul;43(7):3487-90.
doi: 10.1128/JCM.43.7.3487-3490.2005.

Multiplex PCR for identification of seven Streptococcus pneumoniae serotypes targeted by a 7-valent conjugate vaccine

Damien M O'halloran  1 , Mary T Cafferkey
Affiliations

Multiplex PCR for identification of seven Streptococcus pneumoniae serotypes targeted by a 7-valent conjugate vaccine

Damien M O'halloran et al. J Clin Microbiol. 2005 Jul.

Abstract

Here we describe a Streptococcus pneumoniae one-step multiplex PCR assay which identifies by amplicon size the seven capsular polysaccharide serotypes targeted by the 7-valent conjugate vaccine. The multiplex PCR assay was used to blindly assay clinical isolates recovered during 1999 in the Republic of Ireland from cases of invasive disease whose serotypes were previously determined by classical methods.

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Figures

FIG. 1.
FIG. 1.
Gel electrophoresis of purified multiplex PCR amplification products. Lanes 1 and 9, 100-bp DNA ladder (New England Biolabs); lane 2, serotype 9V; lane 3, serotype 4; lane 4, serotype 18C; lane 5, serotype 14; lane 6, serotype 6B; lane 7, serotype 23F; lane 8, serotype 19F.
See this image and copyright information in PMC

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