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. 2005 Jul;43(7):3494-6.
doi: 10.1128/JCM.43.7.3494-3496.2005.

Evaluation of seaFAST, a rapid fluorescent in situ hybridization test, for detection of Helicobacter pylori and resistance to clarithromycin in paraffin-embedded biopsy sections

Julie M Morris  1 , Alisa L Reasonover, Michael G Bruce, Dana L Bruden, Brian J McMahon, Frank D Sacco, Douglas E Berg, Alan J Parkinson
Affiliations

Evaluation of seaFAST, a rapid fluorescent in situ hybridization test, for detection of Helicobacter pylori and resistance to clarithromycin in paraffin-embedded biopsy sections

Julie M Morris et al. J Clin Microbiol. 2005 Jul.

Abstract

A commercially available rapid fluorescent in situ hybridization (FISH) test, (seaFAST H. pylori Combi-Kit; SeaPro Theranostics International, Lelystad, The Netherlands) was used to simultaneously detect the presence of Helicobacter pylori and determine clarithromycin susceptibility in paraffin-embedded biopsy sections. The FISH method was found to be 97% sensitive, 94% specific for the detection of H. pylori and comparable to agar dilution for the detection of resistance to clarithromycin.

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