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Comparative Study
. 2005 Jul;43(7):3504-7.
doi: 10.1128/JCM.43.7.3504-3507.2005.

Evaluation of the COBAS TaqMan 48 real-time PCR system for quantitation of hepatitis B virus DNA

Rafael M Gordillo  1 , Juan Gutiérrez, Manuel Casal
Affiliations
Comparative Study

Evaluation of the COBAS TaqMan 48 real-time PCR system for quantitation of hepatitis B virus DNA

Rafael M Gordillo et al. J Clin Microbiol. 2005 Jul.

Abstract

The purpose of this study is to evaluate the usefulness of the new real-time PCR COBAS TaqMan 48 analyzer, comparing it to the existing COBAS AMPLICOR HBV MONITOR based on conventional PCR technology. The study used 104 samples from different patients. No differences were found in the sensitivity of the tests. There was an excellent correlation between the sample with a viral load within the dynamic range of the two tests (r = 0.938). The COBAS TaqMan test has a wider linear range, and this fact enables quantifying of the viral load without diluting the sample.

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Figures

FIG. 1.
FIG. 1.
Correlation between viral loads detected by the two tests in group A samples (Passing-Bablok and Bland-Altman).
See this image and copyright information in PMC

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