Assessment of soil microbial community structure by use of taxon-specific quantitative PCR assays
- PMID: 16000830
- PMCID: PMC1169028
- DOI: 10.1128/AEM.71.7.4117-4120.2005
Assessment of soil microbial community structure by use of taxon-specific quantitative PCR assays
Abstract
Here we describe a quantitative PCR-based approach to estimating the relative abundances of major taxonomic groups of bacteria and fungi in soil. Primers were thoroughly tested for specificity, and the method was applied to three distinct soils. The technique provides a rapid and robust index of microbial community structure.
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References
-
- Chow, M. L., C. C. Radomski, J. M. McDermott, J. Davies, and P. E. Axelrood. 2002. Molecular characterization of bacterial diversity in Lodgepole pine (Pinus contorta) rhizosphere soils from British Columbia forest soils differing in disturbance and geographic source. FEMS Microbiol. Ecol. 42:347-357. - PubMed
-
- Christensen, M. 1981. Species diversity and dominance in fungal communities, p. 201-232. In D. Wicklow and G. Carroll (ed.), The fungal community: its organization and role in the ecosystem. Marcel Dekker, New York, N.Y.
-
- Cole, J. R., B. Chai, T. L. Marsh, R. J. Farris, Q. Wang, S. A. Kulam, S. Chandra, D. M. McGarrell, T. M. Schmidt, G. M. Garrity, and J. M. Tiedje. 2003. The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Res. 31:442-443. - PMC - PubMed
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