Review
TAT-mediated protein transduction: delivering biologically active proteins to the heart
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Review
TAT-mediated protein transduction: delivering biologically active proteins to the heart
Methods Mol Med.
2005.
Abstract
TAT protein transduction is a novel method of delivering biologically active proteins into cells and tissues through the fusion of a protein transduction domain to the protein of interest. The present chapter outlines the methodology pertaining to the preparation of TAT-fusion proteins and how to efficiently transduce these proteins into cultured cells or isolated rat hearts perfused in Langendorff mode.
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