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Comparative Study
. 2005 Jul 13:6:28.
doi: 10.1186/1471-2296-6-28.

Correlation of same-visit HbA1c test with laboratory-based measurements: a MetroNet study

Kendra L Schwartz  1 , Joseph C Monsur, Monina G Bartoces, Patricia A West, Anne Victoria Neale
Affiliations
Comparative Study

Correlation of same-visit HbA1c test with laboratory-based measurements: a MetroNet study

Kendra L Schwartz et al. BMC Fam Pract. .

Abstract

Background: Glycated hemoglobin (HbA1c) results vary by analytical method. Use of same-visit HbA1c testing methodology holds the promise of more efficient patient care, and improved diabetes management. Our objective was to test the feasibility of introducing a same-visit HbA1c methodology into busy family practice centers (FPC) and to calculate the correlation between the same-visit HbA1c test and the laboratory method that the clinical site was currently using for HbA1c testing.

Methods: Consecutive diabetic patients 18 years of age and older having blood samples drawn for routine laboratory analysis of HbA1c were asked to provide a capillary blood sample for same-visit testing with the BIO-RAD Micromat II. We compared the results of the same-visit test to three different laboratory methods (one FPC used two different laboratories).

Results: 147 paired samples were available for analysis (73 from one FPC; 74 from the other). The Pearson correlation of Micromat II and ion-exchange HPLC was 0.713 (p < 0.001). The Micromat II mean HbA1c was 6.91%, which was lower than the 7.23% from the ion-exchange HPLC analysis (p < 0.001). The correlation of Micromat II with boronate-affinity HPLC was 0.773 (p < 0.001); Micromat II mean HbA1c 6.44%, boronate-affinity HPLC mean 7.71% (p < 0.001). Correlation coefficient for Micromat II and immuno-turbidimetric analysis was 0.927 (p < 0.001); Micromat II mean HbA1c was 7.15% and mean HbA1c from the immuno-turbidimetric analysis was 7.99% (p = 0.002). Medical staff found the same-visit measurement difficult to perform due to the amount of dedicated time required for the test.

Conclusion: For each of the laboratory methods, the correlation coefficient was lower than the 0.96 reported by the manufacturer. This might be due to variability introduced by the multiple users of the Micromat II machine. The mean HbA1c results were also consistently lower than those obtained from laboratory analysis. Additionally, the amount of dedicated time required to perform the assay may limit its usefulness in a busy clinical practice. Before introducing a same-visit HbA1c methodology, clinicians should compare the rapid results to their current method of analysis.

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Figures

Figure 1
Figure 1
Scatterplot and regression line of HbA1c values produced from boronate affinity HPLC and Micromat II.
Figure 2
Figure 2
Scatterplot and regression line of HbA1c values produced from immuno-turbidimetric and Micromat II methods.
Figure 3
Figure 3
Scatterplot and regression line of HbA1c values produced from ion-exchange HPLC and Micromat II methods.
See this image and copyright information in PMC

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