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. 2005 Aug 1;343(1):179-82.
doi: 10.1016/j.ab.2005.03.004. Epub 2005 Mar 30.

Reversed-phase HPLC with UV detection for the determination of N-acetylaspartate and creatine

Mattias Tranberg  1 , Malin H Stridh, Barbro Jilderos, Stephen G Weber, Mats Sandberg
Affiliations

Reversed-phase HPLC with UV detection for the determination of N-acetylaspartate and creatine

Mattias Tranberg et al. Anal Biochem. .
No abstract available

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Figures

Fig. 1
Fig. 1
(A) Chromatogram of a standard of NAA, Cr, and possible interfering compounds (nmol injected in parentheses): 1, hypotaurine (0.5); 2, aspartate (45); 3, NMDA (90); 4, glutamate (45); 5, l-carnosine (0.9); 6, γ-aminobutyric acid (90); 7, Cr (11); 8, pyruvate (18); 9, N-acetylhistidine (1.3); 10, ascorbate (4.5); 11, lactate (99); 12, ATP (1.3); 13, ADP (0.45); 14, α-ketoglutarate (18); 15, NAA (14); 16, GSH (2.7); 17, maleate (0.6); 18, citrate (45); 19, succinate (9); 20, AMP (0.6); 21, N-acetylglutamate (14); 22, fumarate (0.6); 23, urate (4.5); 24, N-acetylaspartylglutamate (2.7); 25, adenosine (2.3); 26, inosine (2.7). Taurine was undetectable at 9 nmol injected. (B) Separation of the Cr and creatinine (Crn) peaks (left) and their different profiles of absorption (right).
Fig. 2
Fig. 2
(A) Chromatogram of UV-absorbing species at 210 nm in an extract of a rat hippocampus. (B) and (C) show the absorbance profiles of the NAA and Cr peaks, respectively, in a standard injection and in an extract of a cultured hippocampal slice from rat.
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References

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