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Comparative Study
. 2005 Jul;11(7):1108-11.
doi: 10.3201/eid1107.041045.

SARS coronavirus detection methods

Susanna K P Lau  1 , Xiao-Yan Che, Patrick C Y Woo, Beatrice H L Wong, Vincent C C Cheng, Gibson K S Woo, Ivan F N Hung, Rosana W S Poon, Kwok-Hung Chan, J S Malik Peiris, Kwok-Yung Yuen
Affiliations
Comparative Study

SARS coronavirus detection methods

Susanna K P Lau et al. Emerg Infect Dis. 2005 Jul.

Abstract

Using clinical samples from patients with severe acute respiratory syndrome, we showed that the sensitivities of a quantitative reverse transcription-polymerase chain reaction (80% for fecal samples and 25% for urine samples) were higher than those of the polyclonal (50% and 5%) and monoclonal (35% and 8%) antibody-based nucleocapsid antigen capture enzyme-linked immunosorbent assays.

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Figures

Figure
Figure
Evaluation of polyclonal and monoclonal antibody–based enzyme-linked immunosorbent assays (ELISAs) for detecting nucleocapsid protein in fecal and urine specimens. The dashed horizontal lines represent the corresponding cutoff optical density values at 450 nm (OD450). SARS, severe acute respiratory syndrome.
See this image and copyright information in PMC

References

    1. Ksiazek TG, Erdman D, Goldsmith CS, Zaki SR, Peret T, Emery S, et al. A novel coronavirus associated with severe acute respiratory syndrome. N Engl J Med. 2003;348:1953–66. 10.1056/NEJMoa030781 - DOI - PubMed
    1. Peiris JS, Chu CM, Cheng VC, Chan KS, Hung IF, Poon LL, et al. Clinical progression and viral load in a community outbreak of coronavirus-associated SARS pneumonia – a prospective study. Lancet. 2003;361:1767–72. 10.1016/S0140-6736(03)13412-5 - DOI - PMC - PubMed
    1. Peiris JS, Yuen KY, Osterhaus AD, Stohr K. The severe acute respiratory syndrome. N Engl J Med. 2003;349:2431–41. 10.1056/NEJMra032498 - DOI - PubMed
    1. Peiris JS, Lai ST, Poon LL, Guan Y, Yam LY, Lim W, et al. Coronavirus as a possible cause of severe acute respiratory syndrome. Lancet. 2003;361:1319–25. 10.1016/S0140-6736(03)13077-2 - DOI - PMC - PubMed
    1. Woo PC, Lau SK, Wong BH, Tsoi HW, Fung AM, Chan KH, et al. Detection of specific antibodies to SARS coronavirus nucleocapsid protein for serodiagnosis of SARS coronavirus pneumonia. J Clin Microbiol. 2004;42:2306–9. 10.1128/JCM.42.5.2306-2309.2004 - DOI - PMC - PubMed

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