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Comparative Study
. 2005 Aug;40(8):536-44.
doi: 10.1097/01.rli.0000170818.03210.ee.

In vitro echogenicity characterization of poly[lactide-coglycolide] (plga) microparticles and preliminary in vivo ultrasound enhancement study for ultrasound contrast agent application

Sonia Lavisse  1 , Angelo Paci, Valerie Rouffiac, Cecile Adotevi, Paule Opolon, Pierre Peronneau, Philippe Bourget, Alain Roche, Michel Perricaudet, Elias Fattal, Nathalie Lassau
Affiliations
Comparative Study

In vitro echogenicity characterization of poly[lactide-coglycolide] (plga) microparticles and preliminary in vivo ultrasound enhancement study for ultrasound contrast agent application

Sonia Lavisse et al. Invest Radiol. 2005 Aug.

Abstract

Objectives: This work includes (1) the characterization of a reproducible poly[lactide-coglycolide] (PLGA) microparticle preparation with an optimial mean diameter and size distribution and (2) the preliminary in vivo ultrasonographic investigation of PLGA microparticles.

Methods: A first series of PLGA microparticle preparations (1 to 15 mum) was acoustically characterized on a hydrodynamic device to select the most appropriate for ultrasound contrast agent application. Preparations of 3-microm microparticles were selected, characterized at different doses, and then injected into 20 melanoma grafted mice for contrast-enhanced power Doppler ultrasonography evaluation.

Results: The 3-microm microparticles (3.26-microm mean diameter with 0.41-microm standard deviation) led to in vitro enhancement of 18.3 dB at 0.62 mg/mL. In vivo experiments showed 47% enhancement of intratumoral vascularization detection after PLGA injection, significantly correlated (P < 0.0001) with preinjection intravascularization and tumoral volume. No toxicity was histologically observed.

Conclusion: The 3-microm PLGA microparticles provided significant enhancement in vitro and in vivo without any toxicity.

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