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. 1992 May;233(1-2):311-4.
doi: 10.1007/BF00587593.

Identification of a nodD-like gene in Frankia by direct complementation of a Rhizobium nodD-mutant

L M Chen  1 , Y H Cui, M Qin, Y L Wang, X L Bai, Q S Ma
Affiliations

Identification of a nodD-like gene in Frankia by direct complementation of a Rhizobium nodD-mutant

L M Chen et al. Mol Gen Genet. 1992 May.

Abstract

Clones from a Frankia At4 gene bank were pooled into groups and mass conjugated into a nodD mutant of Rhizobium leguminosarum bv. viciae by triparental matings. When peas were inoculated with the pooled transconjugants, nodulation was observed. A plasmid, pAt2GX containing Frankia DNA, was isolated from bacteria recovered from these nodules. This plasmid was shown to complement a nodD mutant of R. leguminosarum bv. viciae. Thus pAt2GX contains a Frankia gene that is functionally equivalent to nodD of R. leguminosarum bv. viciae.

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