doi: 10.1016/0378-1119(79)90045-3.
A rightward promoter to the left of the att site of lambda phage DNA: possible participant in site-specific recombination
- PMID: 160359
- DOI: 10.1016/0378-1119(79)90045-3
Item in Clipboard
A rightward promoter to the left of the att site of lambda phage DNA: possible participant in site-specific recombination
Gene.
1979 Nov.
Abstract
The binding has been studied of Escherichia coli RNA-polymerase to the fragments of lambda DNA obtained by digestion with restriction endonucleases BsuI, HindIII, BamHI, EcoRI and HindII + III. There are at least six sites of RNA-polymerase binding in the b2-region. In vitro transcription of those BsuI-fragments of the b2-region which contain six binding sites is rightward. Therefore, the fragments contain promoters rather than mere RNA-polymerase binding sites. One of the promoters of the b2 region named patt was calculated to be about 50 bp to the left of the att site. We postulate that this promoter might correspond to the hef-target which was described as important for the site-specific recombination.
Similar articles
-
[Identification in vivo of promoter activity in the left site of the att region of bacteriophage lambda DNA].Mol Biol (Mosk). 1988 Jan-Feb;22(1):44-54. Mol Biol (Mosk). 1988. PMID: 2836722 Russian.
-
[Restrictase BamHI, HindII, EcoRI and SmaI mapping of hybrid lambda-phi 80 phages with recombination in the structural gene region].Genetika. 1981;17(11):1895-903. Genetika. 1981. PMID: 6274737 Russian.
-
[Localization of the Escherichia coli RNA-polymerase binding site (promotor Patt) on phage lambda DNA near the integration site].Dokl Akad Nauk SSSR. 1982;264(4):999-1001. Dokl Akad Nauk SSSR. 1982. PMID: 6213394 Russian. No abstract available.
-
Phage lambda and the regulation of transcription termination.Cell. 1988 Jan 15;52(1):5-6. doi: 10.1016/0092-8674(88)90523-5. Cell. 1988. PMID: 2449971 Review. No abstract available.
-
A comprehensive molecular map of bacteriophage lambda.Gene. 1979 Nov;7(3-4):217-70. doi: 10.1016/0378-1119(79)90047-7. Gene. 1979. PMID: 160360 Review.
Cited by
-
Analysis of the occurrence of promoter-sites in DNA.Nucleic Acids Res. 1986 Jan 10;14(1):109-26. doi: 10.1093/nar/14.1.109. Nucleic Acids Res. 1986. PMID: 2935785 Free PMC article.
-
Posttranscriptional control of bacteriophage lambda gene expression from a site distal to the gene.Proc Natl Acad Sci U S A. 1982 Jan;79(2):238-42. doi: 10.1073/pnas.79.2.238. Proc Natl Acad Sci U S A. 1982. PMID: 6281759 Free PMC article.
-
Structural bases of a long-stretched deletion: completing the lambda plac5 DNA primary structure.Nucleic Acids Res. 1988 Nov 11;16(21):10199-212. doi: 10.1093/nar/16.21.10199. Nucleic Acids Res. 1988. PMID: 2973573 Free PMC article.
-
RNA polymerase idling and clearance in gal promoters: use of supercoiled minicircle DNA template made in vivo.Proc Natl Acad Sci U S A. 1993 Jan 15;90(2):472-6. doi: 10.1073/pnas.90.2.472. Proc Natl Acad Sci U S A. 1993. PMID: 8380640 Free PMC article.
-
Aberrant regulation of synthesis and degradation of viral proteins in coliphage lambda-infected UV-irradiated cells and in minicells.J Virol. 1987 Oct;61(10):3254-65. doi: 10.1128/JVI.61.10.3254-3265.1987. J Virol. 1987. PMID: 2957511 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
