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. 2005 Aug;49(8):3562-5.
doi: 10.1128/AAC.49.8.3562-3565.2005.

In vivo transfer of plasmid-encoded ACC-1 AmpC from Klebsiella pneumoniae to Escherichia coli in an infant and selection of impermeability to imipenem in K. pneumoniae

Philippe Bidet  1 , Béatrice Burghoffer, Valérie Gautier, Naïma Brahimi, Patricia Mariani-Kurkdjian, Alaa El-Ghoneimi, Edouard Bingen, Guillaume Arlet
Affiliations

In vivo transfer of plasmid-encoded ACC-1 AmpC from Klebsiella pneumoniae to Escherichia coli in an infant and selection of impermeability to imipenem in K. pneumoniae

Philippe Bidet et al. Antimicrob Agents Chemother. 2005 Aug.

Abstract

We describe in vivo selection of a Klebsiella pneumoniae strain with diminished imipenem susceptibility attributable to plasmid-encoded ACC-1 beta-lactamase production and loss of a 36-kDa major outer membrane protein, together with transfer of this plasmid from K. pneumoniae to Escherichia coli in a Tunisian infant.

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Figures

FIG. 1.
FIG. 1.
PFGE analysis. (A) PFGE patterns of SpeI-restricted DNA from K. pneumoniae. Lane 1, isolate K1; lanes 2 and 3, isolate K2; lane 4, outbreak isolate (SLK54) producing ACC-1 β-lactamase (14); lanes 5 to 8, unrelated strains of K. pneumoniae; lane M, molecular weight marker (lambda ladder; Bio-Rad). (B) PFGE patterns of NotI-restricted DNA from E. coli. Lanes 1 to 3, unrelated strains of E. coli; lane 4, isolate E1; lane 5, isolate E2; lane M, molecular weight marker (lambda ladder; Bio-Rad).
FIG. 2.
FIG. 2.
Outer membrane protein profiles of K. pneumoniae clinical isolates K1 and K2 expressing ACC-1. Outer membrane proteins were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis in 12% acrylamide. Lane 1, molecular size standard; lane 2, isolate K1 (imipenem susceptible); lane 3, isolate K2 (imipenem resistant).
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References

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