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Multicenter Study
. 2005 Aug;58(8):894-5.
doi: 10.1136/jcp.2004.021352.

An alternative protocol for DNA extraction from formalin fixed and paraffin wax embedded tissue

R Coura  1 , J C Prolla, L Meurer, P Ashton-Prolla
Affiliations
Multicenter Study

An alternative protocol for DNA extraction from formalin fixed and paraffin wax embedded tissue

R Coura et al. J Clin Pathol. 2005 Aug.

Abstract

Background: DNA extraction from paraffin wax embedded tissue requires special protocols, and most described methods report an amplification success rate of 60-80%.

Aims: To propose a simple and inexpensive protocol consisting of xylene/ethanol dewaxing, followed by a kit based extraction.

Method: Xylene/ethanol dewaxing was followed by a long rehydration step and a kit based DNA extraction step.

Results: This method produced a 100% amplification success rate for fragments of 121 to 227 bp for tamponated formalin fixed paraffin wax embedded tissue.

Conclusion: This cost effective and non-laborious protocol can successfully extract DNA from tamponated formalin fixed paraffin wax embedded tissue and should facilitate the molecular analysis of a large number of archival specimens in retrospective studies.

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Figures

Figure 1
Figure 1
Agarose gel (2%) stained with ethidium bromide, showing the polymerase chain reaction products of the four markers analysed.
See this image and copyright information in PMC

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