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. 2005 Aug 7;11(29):4457-60.
doi: 10.3748/wjg.v11.i29.4457.

Histological and ultrastructural changes induced by selenium in early experimental gastric carcinogenesis

Yan-Ping Su  1 , Jun-Min Tang, Yan Tang, Hui-Ying Gao
Affiliations

Histological and ultrastructural changes induced by selenium in early experimental gastric carcinogenesis

Yan-Ping Su et al. World J Gastroenterol. .

Abstract

Aim: To investigate the effect and significance of selenium in early experimental gastric carcinogenesis.

Methods: Weaning male Wistar rats were divided randomly into normal control group, experiment control group, low selenium (2 mg/L) group and high selenium (4 mg/L) group. Wistar rat gastric carcinogenesis was induced by N-methyl-N-nitro-N-nitroso guanidine (MNNG) (20 mg/kg) gavage daily for 10 d. Na2SeO3 was given by piped drinking 1 wk prior to MNNG gavage. The rats were killed at the 43rd wk. The surface characteristics of gastric mucosa were observed with naked eyes. Histopathologic changes of rat gastric mucosa were observed by HE staining and AB-PAS methods. The changes of cellular ultrastructure were observed under transmission electron microscope. Statistical analysis was carried out by SPSS.

Results: The incidence rate of gastric mucosa erosion, hemorrhage and intestinal metaplasia was 0, 45.5%, 66.7%, and 92.9%, respectively (92.9% vs 45.5%, P < 0.05) in the normal control group, experiment control group, low selenium group, and high selenium group. Leiomyoma formed in the process of inducement of rat gastric carcinoma. Dietary Na2SeO3 (2 and 4 mg/L) slightly increased the incidence rate of leiomyoma (0, 23%, 46.6%, and 46.6%). gastric mucosa did not change in the course of rat gastric carcinogenesis. Dietary Na2SeO3 by pipe drinking could expand the intracellular secretory canaliculus of parietal cells and increase the number of endocrine cells and lysosomes.

Conclusion: Dietary Na2SeO3 by pipe drinking aggravates gastric erosion, hemorrhage and promotes intestinal metaplasia of gastric mucosa. The mechanism may be related with the function of parietal cells.

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Figures

Figure 1
Figure 1
Erosive mucosa of rat stomach (A) and leiomyoma under serosa (B).
Figure 2
Figure 2
Normal mucosa (A) and erosive mucosa (B) of rat stomach, AB-PAS, ×200.
Figure 3
Figure 3
Intracellular secretory canaliculi of parietal cells in normal control group, TEM 5.8×103.
Figure 4
Figure 4
Expansion of intracellular secretory canaliculi of parietal cells in high selenium group, TEM 5.8×103.
Figure 5
Figure 5
Endocrine cells and secondary lysosomes in high selenium group, TEM 10×103.
Figure 6
Figure 6
Infiltration of eosinophils in gastric mucosa of high selenium group, TEM 10×103.
See this image and copyright information in PMC

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