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. 2005 Aug 7;11(29):4583-6.
doi: 10.3748/wjg.v11.i29.4583.

Novel DNA vaccine based on hepatitis B virus core gene induces specific immune responses in Balb/c mice

Yi-Ping Xing  1 , Zu-Hu Huang, Shi-Xia Wang, Jie Cai, Jun Li, Te-Hui W Chou, Shan Lu
Affiliations

Novel DNA vaccine based on hepatitis B virus core gene induces specific immune responses in Balb/c mice

Yi-Ping Xing et al. World J Gastroenterol. .

Abstract

Aim: To investigate the immunogenicity of a novel DNA vaccine, pSW3891/HBc, based on HBV core gene in Balb/c mice.

Methods: A novel DNA vaccine, pSW3891/HBc, encoding HBV core gene was constructed using a vector plasmid pSW3891. Balb/c mice were immunized with either pSW3891/HBc or empty vector DNA via gene gun. IgG anti-HBc responses in mouse sera were demonstrated by ELISA. Specific cytotoxicity of cytotoxic T lymphocytes (CTLs) of mice was quantitatively measured by lactate dehydrogenase release assay.

Results: HBcAg was expressed effectively in 293T cell line transiently transfected with pSW3891/HBc. Strong IgG anti-HBc responses were elicited in mice immunized with pSW3891/HBc. The end-point titers of anti-HBc reached the highest 1:97,200, 4 wk after the third immunization. The specific CTL killing with the highest specific lysis reached 73.25% at effector:target ratio of 20:1 in mice that received pSW3891/HBc DNA vaccine.

Conclusion: pSW3891/HBc vaccination elicits specific anti-HBc response and induces HBc-specific CTL response in immunized Balb/c mice.

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Figures

Figure 1
Figure 1
Digestion of pSW3891/HBc. Marker: λ/Hind III; C6, C7: clone no.
Figure 2
Figure 2
In vitro HBc expression in pSW3891/HBc transiently transfected 293T cells. V: pSW3891; L: lysate; C6: pSW3891/HBc/C6; S: supernatant.
Figure 3
Figure 3
End-point titres of anti-HBc in sera of Balb/c mice immunized with pSW3891/HBc.
Figure 4
Figure 4
Specific cytotoxicity of splenocytes inBalb/c mice immunized with pSW3891/HBc.
See this image and copyright information in PMC

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