Localization of orexin-A-immunoreactive fibers in the mesencephalic trigeminal nucleus of the rat

Abstract

Orexin A is a neuropeptide located exclusively in neurons in the hypothalamic nuclei involved in the central regulation of many brain functions, related to motor activity and state-dependent processes. Orexins modulate behavioral state via actions across multiple terminal fields. In order to determine whether the mesencephalic trigeminal neurons may receive a direct hypothalamic orexinergic input, the distribution of orexin A immunoreactivity was examined in the rat mesencephalic trigeminal nucleus (MTN), using orexin A immunohistochemistry. Orexin-A-immunostained nerve fibers and terminals were found in a close apposition to the perikarya of primary afferent neurons in the MTN with a marked rostrocaudal gradient in their density. In the caudal pontine MTN, only scattered orexin-A-immunoreactive fibers were found, while more rostrally in the pons, and in the midbrain-pontine junction part of the nucleus, orexin-A-immunopositive varicosities were relatively more abundant, located in close proximity to or often surrounding the neuronal profiles. At the level of the inferior or superior colliculi, a large number of orexin-A-containing neuronal processes and terminal arborizations were observed traveling toward and contacting mesencephalic trigeminal neurons, some of which were multipolar. The results of this study show that MTN neurons receive orexin A hypothalamic innervation with a somatotopic arrangement of the projections in the nucleus. The central orexinergic system may exert direct influence upon jaw movements at the level of the MTN and thus to participate in the control of feeding behavior.